Skills
- Microscopy / Imaging: Structured Illumination Microscopy (SIM), Slimfield Microscopy, TIRF, Confocal Microscopy, Light-Sheet Microscopy, Single Particle Tracking, Live-cell imaging, 24h time-lapse imaging
- Sample Preparation: Microtome sectioning, fixation, embedding, mounting, blocking, optical clearing
- Staining / Labeling & Immunology: DAPI, FM 4-64, fluorescent proteins (e.g., sfGFP, mNeon, mVenus, mCherry/RFP), Immunohistochemistry (IHC), ELISA
- Image Processing & Data: ImageJ/FIJI, image segmentation (e.g., watershed), image processing & analysis, Celltool, OMERO server, Zeiss ZEN Blue/Black
- Lab Automation / Screening: Liquid handling station, high-throughput screening, plate reader (Tecan M200)
- Microbiology: Bacteria (e.g., Bacillus subtilis, E. coli), selective media
- Molecular Biology: Gibson Assembly, restriction cloning, SnapGene, NCBI, plasmids, SDS-PAGE / Western blot, transfection / transduction (e.g., RCAS system), RNA extraction / cDNA, qPCR, RT-PCR
- Cell Biology: 2D/3D cell culture (spheroids), iPS cells, ES cells, mammalian cell lines (e.g., U343, T47D, HeLa)
- Flow Cytometry / Cell Sorting: FACS
- Software & Programming: Linux (Debian), Python, Jupyter, R, RStudio, Matlab, SQL, LabView, GitHub, LaTeX, SPSS, Inkscape, VMD, Chimera, RCSB, prototyping
- Machine Learning / AI: Supervised & unsupervised learning, scikit-learn, TensorFlow, PyTorch, Natural Language Processing
- Chemical Analysis: rp-HPLC, natural compound libraries (ENZO)
- Laboratory Practice / Safety: Laboratory work according to biosafety levels S1/S2
International Experience
01/2007 - 03/2008
Round-the-world: Venezuela, Columbia, Argentina, Ecuador, Bolivia, Chile, Peru, Brasil, Australia (work & travel), New Zealand, Singapore, Malaysia
Other countries:
Mexico, Guatemala, Belize, Canada, Sweden, Denmark, Spain, Croatia, France, Poland, Swiss, U.K., Estonia