Data Processing

Capability to handle diverse and large biological data; understanding of linking data; focus on structural biology

Biochemistry

analytical and large-scale purification of recombinant proteins and protein complexes using bacterial, yeast, baculovirus and BacMam expression systems, reconstitution of membrane proteins in nanodisks and liposomes, purification of ribosomes from T. thermophilus, E.coli, S.cerevisiae. Affinity, ion-exchange, size-exclusion and hydrophobic chromatography, HPLC.

Structural Biology

cryoEM grids preparation, cryoEM analysis and data collection (Biotwin, Glacios, Krios), cryoEM data processing (Cryosparc, Relion). Crystallization, X-ray data collection, data processing, structure determination, structure validation, analysis and visualization.

Biophys. methods

sedimentation analysis (analytical ultracentrifugation), dynamic light scattering, nano differential scanning fluorimetry, microscale thermophoresis, filter binding assay, isothermal titration calorimetry, stop-flow FRET technique

Mol. Biology

general biology techniques, cloning, western-blot, luciferase-based reporter assay, RNA purification, tRNA and mRNA-based assays.

Communication

Excellent presentation, written communication, inter-personal and team-player skills, strong problem solving and fast learning skills, high self-motivation, supervision of students.