Capability to handle diverse and large biological data; understanding of linking data; focus on structural biology
analytical and large-scale purification of recombinant proteins and protein complexes using bacterial, yeast, baculovirus and BacMam expression systems, reconstitution of membrane proteins in nanodisks and liposomes, purification of ribosomes from T. thermophilus, E.coli, S.cerevisiae. Affinity, ion-exchange, size-exclusion and hydrophobic chromatography, HPLC.
cryoEM grids preparation, cryoEM analysis and data collection (Biotwin, Glacios, Krios), cryoEM data processing (Cryosparc, Relion). Crystallization, X-ray data collection, data processing, structure determination, structure validation, analysis and visualization.
sedimentation analysis (analytical ultracentrifugation), dynamic light scattering, nano differential scanning fluorimetry, microscale thermophoresis, filter binding assay, isothermal titration calorimetry, stop-flow FRET technique
general biology techniques, cloning, western-blot, luciferase-based reporter assay, RNA purification, tRNA and mRNA-based assays.
Excellent presentation, written communication, inter-personal and team-player skills, strong problem solving and fast learning skills, high self-motivation, supervision of students.
Publications on request
Awards & Fellowships on request