a Randstad company

Biochemie, Strukturbiologie, Biophysik

Profil
Top-Skills
Datenanalyse Biotechnologie Project Controlling Testanalyse
Verfügbar ab
19.12.2022
Noch verfügbar - Schnell sein lohnt sich: Der Experte kann bereits für Projekte vorgesehen sein.
Verfügbar zu
50%
davon vor Ort
50%
Einsatzorte

Städte
Frankfurt am Main (+50km)
PLZ-Gebiete
Remote-Arbeit
möglich
Art des Profiles
Freiberufler / Selbstständiger
Der Experte ist als Einzelperson freiberuflich oder selbstständig tätig.

2 Jahre 4 Monate

2019-07

2021-10

Expression and purification

Postdoctoral Fellow
Rolle
Postdoctoral Fellow
Projektinhalte
Expression and purification of voltage-gated potassium channels, reconstitution in lipid nanoparticles (nanodisks and liposomes), cryoEM analysis, data collection and data processing.
Kunde
Max-Planck Institute of Biophysics, Frankfurt am Main, Germany
1 Jahr 2 Monate

2018-07

2019-08

Optimisation of expression conditions

Senior Scientists I
Rolle
Senior Scientists I
Projektinhalte
Optimisation of expression conditions of proteins and protein complexes in Escherichia coli and baculovirus expression systems, large-scale purification, quality assessment, and crystal structure determination.
Kunde
Selvita S.A., Department of Biochemistry, CRO, Krakow, Poland
5 Jahre 6 Monate

2012-04

2017-09

X-ray crystallography

Postdoctoral Fellow
Rolle
Postdoctoral Fellow
Projektinhalte
X-ray crystallography of translation inhibitors bound to eukaryotic 80S ribosome.
Kunde
Institute of Genetics, Molecular and Cellular Biology, Strasbourg, France
1 Monat

2015-02

2015-02

Contributed to the establishment

Co-inventor
Rolle
Co-inventor
Projektinhalte
Contributed to the establishment by development of crystal structure analysis of interactions with translation inhibitors.
Kunde
on request, Strasbourg, France
6 Monate

2011-10

2012-03

In vivo screening

Junior Research Scientist
Rolle
Junior Research Scientist
Projektinhalte
In vivo screening of translation inhibitors by the reporter assay.
Kunde
Moscow State University, Russia
3 Jahre

2008-10

2011-09

Investigation of the functional role of the methylation

Ph.D. Fellow
Rolle
Ph.D. Fellow
Projektinhalte
Investigation of the functional role of the methylation of G966/C967 in 16S rRNA Escherichia coli.
Kunde
Moscow State University, Russia
5 Monate

2010-01

2010-05

Applied FRET-based stopped flow technique

Trainee
Rolle
Trainee
Projektinhalte
Applied FRET-based stopped flow technique to study the impact of m²G966/m?C96 methylation on the kinetics of translation initiation.
Kunde
Max-Planck Institute for Biophysical Chemistry, Goettingen, Germany

1 Monat

2012-03

2012-03

Ph.D.

Ph.D. Degree in Chemistry, Moscow State University, Russia
Abschluss
Ph.D. Degree in Chemistry
Institution, Ort
Moscow State University, Russia
4 Jahre 10 Monate

2003-09

2008-06

Diploma

Diploma in Chemistry, Moscow State University, Russia
Abschluss
Diploma in Chemistry
Institution, Ort
Moscow State University, Russia

Russian native
English fluent, professional
French intermediate
German beginner

Top Skills
Datenanalyse Biotechnologie Project Controlling Testanalyse
Produkte / Standards / Erfahrungen / Methoden

Data Processing

Capability to handle diverse and large biological data; understanding of linking data; focus on structural biology


Biochemistry

analytical and large-scale purification of recombinant proteins and protein complexes using bacterial, yeast, baculovirus and BacMam expression systems, reconstitution of membrane proteins in nanodisks and liposomes, purification of ribosomes from T. thermophilus, E.coli, S.cerevisiae. Affinity, ion-exchange, size-exclusion and hydrophobic chromatography, HPLC.


Structural Biology

cryoEM grids preparation, cryoEM analysis and data collection (Biotwin, Glacios, Krios), cryoEM data processing (Cryosparc, Relion). Crystallization, X-ray data collection, data processing, structure determination, structure validation, analysis and visualization.


Biophys. methods

sedimentation analysis (analytical ultracentrifugation), dynamic light scattering, nano differential scanning fluorimetry, microscale thermophoresis, filter binding assay, isothermal titration calorimetry, stop-flow FRET technique


Mol. Biology

general biology techniques, cloning, western-blot, luciferase-based reporter assay, RNA purification, tRNA and mRNA-based assays.


Communication

Excellent presentation, written communication, inter-personal and team-player skills, strong problem solving and fast learning skills, high self-motivation, supervision of students.

Publications on request

Awards & Fellowships on request

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